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LiCl Wash buffer

Recipe  
Tris HCl pH 8 10 mM
LiCl 250 mM
NP40 0.5 %
EDTA pH 8 1mM
Preparation for 50 ml
Tris HCl 1M pH 8 0.5 ml
LiCl 5M 2.5 ml
NP40 0,25 ml
EDTA 0.5M pH 8 0.1 ml
H20 46.65 ml