Lysis Buffer
| Recipe | ||
|---|---|---|
| Hepes pH 7.5 | 50 mM | |
| NaCl | 140 mM | |
| EDTA pH 8 | 1 mM | |
| Triton X-100 | 1 % | |
| Sodium deoxycholate | 0.1 % | |
| Proteases inhibitor (1 tab/2ml PBS) 25x | 1X | Prepare extemp |
| CaCl2 | 2 mM | Prepare extemp |
| Recipe | ||
|---|---|---|
| Hepes pH 7.5 | 50 mM | |
| NaCl | 140 mM | |
| EDTA pH 8 | 1 mM | |
| Triton X-100 | 1 % | |
| Sodium deoxycholate | 0.1 % | |
| Proteases inhibitor (1 tab/2ml PBS) 25x | 1X | Prepare extemp |
| CaCl2 | 2 mM | Prepare extemp |