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Lysis Buffer

Recipe    
Hepes pH 7.5 50 mM  
NaCl 140 mM  
EDTA pH 8 1 mM  
Triton X-100 1 %  
Sodium deoxycholate 0.1 %  
Proteases inhibitor (1 tab/ml PBS) 20 µl/ml Prepare extemp
CaCl2 2 mM Prepare extemp