Lysis Buffer NaCl 0.5 M
| Recipe | |
|---|---|
| Hepes pH 7.5 | 50 mM |
| NaCl | 500 mM |
| EDTA pH 8 | 1 mM |
| Triton X-100 | 1 % |
| Sodium deoxycholate | 0.1 % |
| Preparation | for 50 ml |
|---|---|
| Hepes 1M pH 7.5 | 2.5 ml |
| NaCl 5M | 5 ml |
| EDTA 0.5M pH 8 | 0.1 ml |
| Triton X-100 | 0.5 ml |
| Sodium deoxycholate 5% | 1 ml |
| H20 | 40.9 ml |