Solutions

List

List of solutions to prepare
Tris HCl 1 M pH 8
EDTA 0.5 M pH 8
EGTA 0.5 M pH 8
HEPES 1 M pH 7.5
Sodium Acetate 3 M pH 5.2
Sodium Deoxycholate 5%
NaCl 5M
LiCl 5M
CaCl2 1M
Glycine 2.5M
PBS
List of solutions to buy
SDS 20%
Formaldehyde 37%
Triton-X100
NP-40
Phenol/Chloroform
Glycogen
Ethanol

Recipes

Tris HCl, 1M, pH8

12.114 g of tris [hydroxymethyl] aminomethane in ~80ml of H2O
Adjust pH with HCl
H2O qs 100ml
Autoclave

EDTA 0.5M pH8

18.61 g of EDTA in ~80ml of H2O
Adjust pH with NaOH (~2g)
H2O qs 100ml
Autoclave

EGTA 0.5M pH8

9.5 g of EDTA in ~30ml of H2O
Adjust pH with NaOH (~2g)
H2O qs 50ml

HEPES 1M pH 7.5

23.83g of HEPES in ~80ml of H2O
Adjust pH with NaOH
H2O qs 100ml
Filtersterilze

Sodium Acetate 3M pH5.2

40.8 g of Sodium Acetate (trihydrate) in ~70ml of H2O
Adjust pH with Acetic acid 100%
H2O qs 100ml
Filtersterilze

NaCl 5M

29.22g in 100ml H2O

LiCl 5M

21.2g in 100ml H2O

CaCl2 1M

14.7g in 100ml H2O

Glycine 2.5M

18.77g in 100ml H2O

Sodium deoxycholate 5%

5g in 100ml H2O