Other reagents
MNase
Micrococcal nuclease NEB #M0247
Antibodies
Target | Supplier | Catalog Number | Lot Number | Quantity per IP |
---|---|---|---|---|
H3K36me3 | Abcam | ab9050 | GR3307136-2 | 10 µL |
H3K27me3 | Sigma | 17-622 | 3504435 | 5µL |
H3K9me3 | Abcam | ab8898 | GR3373564-1 | 3.75µL (=3.75µg) |
H3K4me3 | ActiveMotif | 39159 | 31420006 | 4 µL |
H4K20me3 | ActiveMotif | 39671 | 35518005 | 7.5 µL |
RNA Pol.II | ActiveMotif | 91151 | 26520003 | 4 µL (=4µg) |
GFP | Abcam | ab290 | GR3321614-1 | 3µL |
Magnetic beads
Dynabeads protein A Invitrogen 10001D
Vortex (> 30sec) the beads.
Then, the beads must be washed before use:
Aliquot the appropriate volume of beads and place on the magntic rack
Wash with Lysis buffer, 1 ml / 250µl of beads, 3 min, at RT
Collect the beads and resuspend in Lysis buffer, same volume as initially
Store at 4°C