Other reagents

MNase

Micrococcal nuclease NEB #M0247

Antibodies

Target	Supplier	Catalog Number	Lot Number	Quantity per IP
H3K36me3	Abcam	ab9050	GR3307136-2	10 µL
H3K27me3	Sigma	17-622	3759744	5µL
H3K9me3	Abcam	ab8898	GR3373564-1	3.75µL (=3.75µg)
H3K4me3	ActiveMotif	39159	31420006	4 µL
H4K20me3	ActiveMotif	39671	35518005	7.5 µL
RNA Pol.II S5	Abcam	ab5131	GR3436140-1	2.5 µL (=1.5µg)
GFP	Abcam	ab290	GR3321614-1	3µL

Magnetic beads

Dynabeads protein A Invitrogen 10001D

Vortex (> 30sec) the beads.

Then, the beads must be washed before use:\

Aliquot 250 µl of beads and place on the magnetic rack
Wash with Lysis buffer, 1 ml / 250 µl of beads, 3 min, at RT
Collect the beads and resuspend in 250 µl Lysis buffer
Store at 4°C\

NB: the volume of beads you wash can be scaled according to your needs