ChIPseq Workshop

This workshop is part of the genE² master of the University Paris-Saclay

With the support of :

Organized by:
Benoit Moindrot and Pierre Grognet

The worshop will take place from the 16th to the 20th of September 2024

Here is the schedule of the workshop

Introduction to Epigenetics and Epigenomics

• Epigenomics

Introduction to ChIP technic

• ChIP description

About Podospora anserina and its genome

• More about Podospora anserina
• Podospora anserina’s genome

ChIP Protocol

• ChIP protocol
• QuBit protocol

qPCR quantifications

• qPCR on ChIPed chromatin

Library preparation

• Library preparation protocol

Reagents:

• Reagents

Buffers recipes:

• Lysis buffer
• Lysis buffer NaCl
• LiCl Washbuffer
• TES
• Tris-EDTA
• Tris HCl 10 mM
• EGTA 0.5M pH8

Reports:

• Information on the reports